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Paper   IPM / Nano-Sciences / 16075
School of Nano Science
  Title:   Phototherapy alters the oncogenic metabolic activity of breast cancer cells
1.  Mehdi Shakibaie
2.  Maryam Vaezjalalib
3.  Hashem Rafii Tabar
4.  Pezhman Sasanpour
  Status:   Published
  Journal: Photodiagnosis and Photodynamic Therapy
  Vol.:  30
  Year:  2020
  Pages:   101695
  Publisher(s):   Elsevier. B. V
  Supported by:  IPM
Background: Metabolic reprogramming in cancer cells is a strategy to attain a high proliferation rate, invasion, and metastasis. In this study, the effects of phototherapy at different wavelengths were investigated on the metabolic activity of breast cancer cells. Methods: The states of the MCF7 cells proliferation and viability were measured by the MTT assay. Glucose consumption and the lactate formation in the LED-irradiated cells culture were analyzed by biochemical assay kits. The Amino acid concentration in the culture media of the MCF7 cells was analyzed using HPLC. Moreover, the gene expression of some glycolytic, TCA cycle and pentose phosphate cycleenzymes were assessed by real time PCR. Results: Phototherapy at wavelength of 435 nm decreased the cell viability by 23diminished respectively by 22lactate formation were increased in the 629nm irradiated cells by 112irradiation decreased the glutamine consumption while the red light increased it in comparison with the control group.

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