“School of Physics”

Back to Papers Home
Back to Papers of School of Physics

Paper   IPM / P / 15311
School of Physics
  Title:   Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns
1.  M. Zandieh
2.  S. N. Hosseini
3.  M. Vossoughi
4.  M. Khatami
5.  S. Abbasian
6.  A. Moshaii
  Status:   Published
  Journal: Analytical Biochemistry
  Vol.:  548
  Year:  2018
  Pages:   96-101
  Supported by:  IPM
This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes that LSPR biosensing can be considered as a sensitive, simple, and label-free method for endotoxin detection in the quality control laboratories.

Download TeX format
back to top
scroll left or right