“School of Nano-Sciences”
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| Paper IPM / Nano-Sciences / 15209 |
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In this paper, oblique illumination is used to improve the lateral resolution and edge sharpness in microsphere-assisted Mirau digital holographic microscopy (Mirau-DHM). E. Abbe showed that tilting the illumination light allows entrance of higher spatial frequencies into the imaging system thus increasing the resolution power. We extended the idea to common-path DHM, based on Mirau objective, toward super-resolved 3D imaging. High magnification Mirau objectives are very expensive and low magnification ones suffer from low resolution, therefore, any attempt to increase the effective resolution of the system may be of a great interest. We have already demonstrated the effective resolution increasing of a Mirau-DHM system by incorporating a transparent microsphere within the working distance of the objective. Here, we show that by integrating a microsphere-assisted Mirau-DHM with the oblique illumination even higher resolutions can be achieved. We have applied the technique for various samples and have shown the increase in the lateral resolution for the both cases of Mirau-DHM with and without the microsphere.
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