Biological molecules constituted charges are distributed in certain manner and their electrostatic short range and long range interactions define their functional structure and dynamics. Detection and recording of the resulting continuous transitional motions and forces and fields created at sub-nanoscale levels in real time are beyond the capability of the most of current instruments. Consequently, to work out the mechanism(s) involved at atomic or molecular levels, we have to use rather macroscopic experimentally gathered data and feed them into algorithms and equations defined based on the physico-chemical laws. Thus, although, most of the recorded events occurred for a reason that should be revealed by the cause and effect laws in a deterministic manner, there are cases where they occur at atomic and subatomic levels and time scales of femtoseconds or less, that we are not able to record them all. Consequently, as we are not able to monitor and detect the real characteristics of fast and weak events, we take the stochastic approaches to explain them. Accordingly, the statistical analysis of only few recorded points out of all that occurred, are used to formulate the event and to propose the status of thousand to millions of the events occurred in between.

Current gating and activities of nano-channel forming proteins is the result of a spectrum of slow to ultrafast dynamics of their constituent atoms and groups located in the vicinity of their conducting path, in contact with water and translocating molecule(s), as well as those situated far away. The channel gating of voltage sensitive ion channels is the result of many factors including; bioelectric profile of biological water in the bulk and opening filters of the channel, along the conducting path, cross sectional and longitudinal electric, magnetic and electromagnetic fields set across the channel, at the constriction zone or selecting area and arrangements, hydration status and dynamics of individual amino acid side chains and their constituent exposed and non-exposed atoms at different parts of channel.
Here, the experimental current recordings of single ion channel in real time and the effects of pH, Pd, ionic strength, temperature and applied EMF is presented and their deterministic or stochastic nature is justified and means of proper analysis and formulation is discussed.


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